Comparative study of nutritional mode and mycorrhizal fungi in green and albino variants of Goodyera velutina, an orchid mainly utilizing saprotrophic rhizoctonia.
Identifieur interne : 000611 ( Main/Exploration ); précédent : 000610; suivant : 000612Comparative study of nutritional mode and mycorrhizal fungi in green and albino variants of Goodyera velutina, an orchid mainly utilizing saprotrophic rhizoctonia.
Auteurs : Kenji Suetsugu [Japon] ; Masahide Yamato [Japon] ; Jun Matsubayashi [Japon] ; Ichiro Tayasu [Japon]Source :
- Molecular ecology [ 1365-294X ] ; 2019.
Descripteurs français
- KwdFr :
- Espaceur de l'ADN ribosomique (génétique), Fonctions de vraisemblance (MeSH), Isotopes de l'azote (MeSH), Isotopes du carbone (MeSH), Marquage isotopique (MeSH), Mycorhizes (physiologie), Orchidaceae (microbiologie), Phylogenèse (MeSH), Phénomènes physiologiques nutritionnels (MeSH), Rhizoctonia (physiologie).
- MESH :
- génétique : Espaceur de l'ADN ribosomique.
- microbiologie : Orchidaceae.
- physiologie : Mycorhizes, Rhizoctonia.
- Fonctions de vraisemblance, Isotopes de l'azote, Isotopes du carbone, Marquage isotopique, Phylogenèse, Phénomènes physiologiques nutritionnels.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Ribosomal Spacer.
- chemical : Carbon Isotopes, Nitrogen Isotopes.
- microbiology : Orchidaceae.
- physiology : Mycorrhizae, Rhizoctonia.
- Isotope Labeling, Likelihood Functions, Nutritional Physiological Phenomena, Phylogeny.
Abstract
The majority of chlorophyllous orchids form mycorrhizal associations with so-called rhizoctonia fungi, a phylogenetically heterogeneous assemblage of predominantly saprotrophic fungi in Ceratobasidiaceae, Tulasnellaceae, and Serendipitaceae. It is still a matter of debate whether adult orchids mainly associated with rhizoctonia species are partially mycoheterotrophic. Here, we investigated the nutritional modes of green and albino variants of Goodyera velutina, an orchid species considered to be mainly associated with Ceratobasidium spp., by measuring their 13 C and 15 N abundances, and by molecular barcoding of their mycorrhizal fungi. Molecular analysis revealed that both green and albino variants of G. velutina harbored a similar range of mycobionts, mainly saprotrophic Ceratobasidium spp., Tulasnella spp., and ectomycorrhizal Russula spp. In addition, stable isotope analysis revealed that albino variants were significantly enriched in 13 C but not so greatly in 15 N, suggesting that saprotrophic Ceratobasidium spp. and Tulasnella spp. are their main carbon source. However, in green variants, 13 C levels were depleted and those of 15 N were indistinguishable from the co-occurring autotrophic plants. Therefore, we concluded that the albino G. velutina variants are fully mycoheterotrophic plants whose C derives mainly from saprotrophic rhizoctonia, while the green G. velutina variants are mainly autotrophic plants, at least at our study site, in spite of their additional associations with ectomycorrhizal fungi. This is the first report demonstrating that adult nonphotosynthetic albino variants can obtain their nutrition mainly from nonectomycorrhizal rhizoctonia.
DOI: 10.1111/mec.15213
PubMed: 31448451
Affiliations:
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sortKey="Suetsugu, Kenji" sort="Suetsugu, Kenji" uniqKey="Suetsugu K" first="Kenji" last="Suetsugu">Kenji Suetsugu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Graduate School of Science, Kobe University, Kobe, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biology, Graduate School of Science, Kobe University, Kobe</wicri:regionArea><wicri:noRegion>Kobe</wicri:noRegion></affiliation></author><author><name sortKey="Yamato, Masahide" sort="Yamato, Masahide" uniqKey="Yamato M" first="Masahide" last="Yamato">Masahide Yamato</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Education, Chiba University, Chiba, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Education, Chiba University, Chiba</wicri:regionArea><wicri:noRegion>Chiba</wicri:noRegion></affiliation></author><author><name sortKey="Matsubayashi, Jun" sort="Matsubayashi, Jun" uniqKey="Matsubayashi J" first="Jun" last="Matsubayashi">Jun Matsubayashi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biogeochemistry, Japan Agency for Marine-Earth Science and Technology, Yokosuka, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biogeochemistry, Japan Agency for Marine-Earth Science and Technology, Yokosuka</wicri:regionArea><wicri:noRegion>Yokosuka</wicri:noRegion></affiliation></author><author><name sortKey="Tayasu, Ichiro" sort="Tayasu, Ichiro" uniqKey="Tayasu I" first="Ichiro" last="Tayasu">Ichiro Tayasu</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute for Humanity and Nature, Kyoto, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Research Institute for Humanity and Nature, Kyoto</wicri:regionArea><wicri:noRegion>Kyoto</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular ecology</title><idno type="eISSN">1365-294X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbon Isotopes (MeSH)</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Isotope Labeling (MeSH)</term><term>Likelihood Functions (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Nitrogen Isotopes (MeSH)</term><term>Nutritional Physiological Phenomena (MeSH)</term><term>Orchidaceae (microbiology)</term><term>Phylogeny (MeSH)</term><term>Rhizoctonia (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Espaceur de l'ADN ribosomique (génétique)</term><term>Fonctions de vraisemblance (MeSH)</term><term>Isotopes de l'azote (MeSH)</term><term>Isotopes du carbone (MeSH)</term><term>Marquage isotopique (MeSH)</term><term>Mycorhizes (physiologie)</term><term>Orchidaceae (microbiologie)</term><term>Phylogenèse (MeSH)</term><term>Phénomènes physiologiques nutritionnels (MeSH)</term><term>Rhizoctonia (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbon Isotopes</term><term>Nitrogen Isotopes</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Espaceur de l'ADN ribosomique</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Orchidaceae</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Mycorhizes</term><term>Rhizoctonia</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term><term>Rhizoctonia</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Isotope Labeling</term><term>Likelihood Functions</term><term>Nutritional Physiological Phenomena</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fonctions de vraisemblance</term><term>Isotopes de l'azote</term><term>Isotopes du carbone</term><term>Marquage isotopique</term><term>Phylogenèse</term><term>Phénomènes physiologiques nutritionnels</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The majority of chlorophyllous orchids form mycorrhizal associations with so-called rhizoctonia fungi, a phylogenetically heterogeneous assemblage of predominantly saprotrophic fungi in Ceratobasidiaceae, Tulasnellaceae, and Serendipitaceae. It is still a matter of debate whether adult orchids mainly associated with rhizoctonia species are partially mycoheterotrophic. Here, we investigated the nutritional modes of green and albino variants of Goodyera velutina, an orchid species considered to be mainly associated with Ceratobasidium spp., by measuring their <sup>13</sup> C and <sup>15</sup> N abundances, and by molecular barcoding of their mycorrhizal fungi. Molecular analysis revealed that both green and albino variants of G. velutina harbored a similar range of mycobionts, mainly saprotrophic Ceratobasidium spp., Tulasnella spp., and ectomycorrhizal Russula spp. In addition, stable isotope analysis revealed that albino variants were significantly enriched in <sup>13</sup> C but not so greatly in <sup>15</sup> N, suggesting that saprotrophic Ceratobasidium spp. and Tulasnella spp. are their main carbon source. However, in green variants, <sup>13</sup> C levels were depleted and those of <sup>15</sup> N were indistinguishable from the co-occurring autotrophic plants. Therefore, we concluded that the albino G. velutina variants are fully mycoheterotrophic plants whose C derives mainly from saprotrophic rhizoctonia, while the green G. velutina variants are mainly autotrophic plants, at least at our study site, in spite of their additional associations with ectomycorrhizal fungi. This is the first report demonstrating that adult nonphotosynthetic albino variants can obtain their nutrition mainly from nonectomycorrhizal rhizoctonia.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31448451</PMID><DateCompleted><Year>2020</Year><Month>06</Month><Day>11</Day></DateCompleted><DateRevised><Year>2020</Year><Month>06</Month><Day>11</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-294X</ISSN><JournalIssue CitedMedium="Internet"><Volume>28</Volume><Issue>18</Issue><PubDate><Year>2019</Year><Month>09</Month></PubDate></JournalIssue><Title>Molecular ecology</Title><ISOAbbreviation>Mol Ecol</ISOAbbreviation></Journal><ArticleTitle>Comparative study of nutritional mode and mycorrhizal fungi in green and albino variants of Goodyera velutina, an orchid mainly utilizing saprotrophic rhizoctonia.</ArticleTitle><Pagination><MedlinePgn>4290-4299</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/mec.15213</ELocationID><Abstract><AbstractText>The majority of chlorophyllous orchids form mycorrhizal associations with so-called rhizoctonia fungi, a phylogenetically heterogeneous assemblage of predominantly saprotrophic fungi in Ceratobasidiaceae, Tulasnellaceae, and Serendipitaceae. It is still a matter of debate whether adult orchids mainly associated with rhizoctonia species are partially mycoheterotrophic. Here, we investigated the nutritional modes of green and albino variants of Goodyera velutina, an orchid species considered to be mainly associated with Ceratobasidium spp., by measuring their <sup>13</sup> C and <sup>15</sup> N abundances, and by molecular barcoding of their mycorrhizal fungi. Molecular analysis revealed that both green and albino variants of G. velutina harbored a similar range of mycobionts, mainly saprotrophic Ceratobasidium spp., Tulasnella spp., and ectomycorrhizal Russula spp. In addition, stable isotope analysis revealed that albino variants were significantly enriched in <sup>13</sup> C but not so greatly in <sup>15</sup> N, suggesting that saprotrophic Ceratobasidium spp. and Tulasnella spp. are their main carbon source. However, in green variants, <sup>13</sup> C levels were depleted and those of <sup>15</sup> N were indistinguishable from the co-occurring autotrophic plants. Therefore, we concluded that the albino G. velutina variants are fully mycoheterotrophic plants whose C derives mainly from saprotrophic rhizoctonia, while the green G. velutina variants are mainly autotrophic plants, at least at our study site, in spite of their additional associations with ectomycorrhizal fungi. This is the first report demonstrating that adult nonphotosynthetic albino variants can obtain their nutrition mainly from nonectomycorrhizal rhizoctonia.</AbstractText><CopyrightInformation>© 2019 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Suetsugu</LastName><ForeName>Kenji</ForeName><Initials>K</Initials><Identifier Source="ORCID">0000-0002-7943-4164</Identifier><AffiliationInfo><Affiliation>Department of Biology, Graduate School of Science, Kobe University, Kobe, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yamato</LastName><ForeName>Masahide</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Faculty of Education, Chiba University, Chiba, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Matsubayashi</LastName><ForeName>Jun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biogeochemistry, Japan Agency for Marine-Earth Science and Technology, Yokosuka, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tayasu</LastName><ForeName>Ichiro</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Research Institute for Humanity and Nature, Kyoto, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>LC458752</AccessionNumber><AccessionNumber>LC458756</AccessionNumber><AccessionNumber>DQ200924</AccessionNumber><AccessionNumber>LC458758</AccessionNumber><AccessionNumber>LC458759</AccessionNumber><AccessionNumber>LC459956</AccessionNumber><AccessionNumber>LC480733</AccessionNumber><AccessionNumber>LC480736</AccessionNumber><AccessionNumber>MUV66439</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>09</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Ecol</MedlineTA><NlmUniqueID>9214478</NlmUniqueID><ISSNLinking>0962-1083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002247">Carbon Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009587">Nitrogen Isotopes</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002247" MajorTopicYN="N">Carbon Isotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007553" MajorTopicYN="N">Isotope Labeling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016013" MajorTopicYN="N">Likelihood Functions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009587" MajorTopicYN="N">Nitrogen Isotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009747" MajorTopicYN="Y">Nutritional Physiological Phenomena</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029595" MajorTopicYN="N">Orchidaceae</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012232" MajorTopicYN="N">Rhizoctonia</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">13C natural abundance</Keyword><Keyword MajorTopicYN="Y">15N natural abundance</Keyword><Keyword MajorTopicYN="Y">Orchidaceae</Keyword><Keyword MajorTopicYN="Y">autotrophy</Keyword><Keyword MajorTopicYN="Y">mycoheterotrophy</Keyword><Keyword MajorTopicYN="Y">mycorrhiza</Keyword><Keyword MajorTopicYN="Y">rhizoctonia</Keyword><Keyword MajorTopicYN="Y">vegetative albino</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>02</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>07</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>07</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>8</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>6</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>8</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31448451</ArticleId><ArticleId IdType="doi">10.1111/mec.15213</ArticleId></ArticleIdList><ReferenceList><Title>REFERENCES</Title><Reference><Citation>Altschul, S. 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